Microfluidic Cell Processing for Personalized and Regenerative Medicine
15 Sep 2021 | Contributor(s):: Stone, Nicholas Edwin
Advisors: Todd Sulchek, John McDonald, Alexander Alexeev, Ross Ethier, Budd TuckerCell processing lies at the heart of modern medicine. While in the past, the treatment of diseases such as cancer was based mostly on the cancer's tissue of origin and histological classification, today these...
Convective Intracellular Macromolecule Delivery for Cell Engineering Applications
10 Jun 2021 | Contributor(s):: Liu, Anna
Advisors: Todd Sulchek, Krishnendu Roy, Edmund K. Waller, Alexander Alexeev, Mark PrausnitzEfficient intracellular delivery of target macromolecules remains a major obstacle in cell engineering, cell labeling, and other biomedical applications. Current standard methods of intracellular delivery,...
Development of a Microengineered Human Blood-Brain Barrier Model With 3D Astrocytic Network
11 Jan 2021 | Contributor(s):: Ahn, Song Ih
Advisors: YongTae Kim, Allan I. Levey, Mark R. Prausnitz, Shuichi Takayama, Levi WoodThe blood-brain barrier (BBB), a unique vascular border in the central nervous system (CNS), has a highly selective barrier function that prevents unwanted substances from entering the brain. To deliver drugs...
Magnetic Artificial Cilia for Microfluidic Applications
14 Jan 2020 | Contributor(s):: Gowranga Hanasoge, Srinivas Kumar
Advisors: Peter J Hesketh, Alexander Alexeev, Todd Sulchek, Hang Lu, Amanada StocktonBiological organisms use hair-like cilia and flagella to perform fluid manipulations essential for their function and survival. This work aims at developing synthetic analogs of biological cilia. The fabrication,...
Microfluidics for Translating Multifunctional Nanomaterials
14 Jan 2020 | Contributor(s):: Sei, Yoshitaka John
Advisors: YongTae Kim, Julie Champion, James B Dixon, Andres Garcia, Hanjoong JoConflicting results between benchtop experiments, animal models, and clinical trials have posed a significant challenge in translational medical research, motivating the development of technologies with which to...
Microfluidic Generation of Cancer Nanomedicines
14 Jan 2020 | Contributor(s):: Hasani Sadrabadi, Mohammad Mahdi
Advisors: Karl I Jacob, Mostafa A El-Sayed, Luke Brewster, Krishnendu Roy, J. Brandon DixonCancer diagnosis and therapy are perhaps the most promising areas for nanotechnology in medicine, and are expected to soon have applications in the market. The goal of this dissertation was to develop a...
Adhesion Signature Based Enrichment of Tumor Initiating Cells
21 Aug 2019 | Contributor(s):: Cermeno, Efrain
Advisors: Andres J Garcia, Susan N Thomas, Emina H Huang, Hang Lu, Todd C McDevittIn spite of major therapeutic advances, cancer relapse and low rates of patient response to cancer therapeutics persist. This failure is due in part to a small subpopulation of tumor initiating cells (TICs) with...
High Precision Control of Integrated Microfluidics for Nanomanufacturing and Fluidic Sampling
29 May 2019 | Contributor(s):: Toth, Michael J
Advisors: YongTae Kim, Gang Bao, Jun Ueda, Thomas Kurfess, Peter J HeskethCombining the engineering principles of system dynamics and control theory with biological applications of nanoparticle synthesis and organ-on-a-chip, this work aims to advance these areas of research by developing...
High Throughput Multi-Modal Microfluidic System for Isolation of Blood Cells
20 Aug 2018 | Contributor(s):: Tasadduq, Bushra
Advisors: Todd Sulchek, Ali Fatih Sarioglu, Alexander Alexeev, Oliver Brand, Wilbur A. Lam, F. Levent DegertekinIn this study, we have developed a novel, multimodal microfluidic platform for cell sorting which utilizes size and adhesion as label-free biomarkers. The size biomarker is chosen as it...
Cellular Stiffness as a Sorting-Compatible Indicator of Stem Cell Potency
22 Jan 2018 | Contributor(s):: Bongiorno, Thomas
Advisors: Todd Sulchek, Wilbur A. Lam, Todd C. McDevitt, John F. McDonald, Paula M. VertinoDue to their characteristic properties of self-renewal and differentiation, stem cells hold the capacity to serve as phenotype-specific cell factories for various regenerative medicine and tissue...
Mixing and Sampling in a Microfluidic Channel Using Rotating Magnetic Microbeads
22 Aug 2016 | Contributor(s):: Owen, Drew
Advisors: Peter HeskethThis work presents a novel microsystem utilizing an array of rotating magnetic beads inside a microfluidic channel. The magnetic beads are actuated via an external rotating magnetic field dynamically magnetizing small (80% of particles that pass through the 400 um array....
A Microfabricated Rapid Desalting Device for Integration With Electrospraying Tip
27 Aug 2014 | Contributor(s):: Tibavinsky, Ivan Andres
Advisors: Andrei G. Fedorov, Peter Kottke, Peter HeskethElectrospray Ionization (ESI) is a technique that permits the soft ionization of large proteins and biomolecules without fragmenting them, which allows them to be characterized via Mass Spectrometry (MS). It has the potential of permitting...